Application Note: RapidVap Vertex Evaporator

Protocol:

US EPA Method 550.1 requires an evaporation or concentration step when extracting polyaromatic hydrocarbons from liquid.The data presented are compounds from the matrix spike.

Provisions:

RapidVap Vertex Evaporator (Cat#7320020/SN-111150275 A) with 40 mL block.

40 mL I-Chem vials with PTFE lined septa.

Chromatographic grade Methylene Chloride and Acetonitrile.

Agilent 1100 HPLC with Ultraviolet and Fluorescence Detection

Polyaromatic Hydrocarbon standards purchase from Supelco Analytical.

Procedure:

• 40mL vials were filled with 10.0mL of methylene chloride spiked with 5ng/mL – 100ng/mL depending on the concentration of the analytical standard and concentrated down to 1mL.
• The block preheat and temperature was 40°C with 10 p.s.i.of nitrogen.The 0.5mL was brought to 3mL with acetonitrile and then concentrated to 0.5mL.
• Three preparations were injected in duplicate and compared to a 1ug/mL standard of polyaromatic hydrocarbons for percent recovery determinations.

Table 1(below) shows the recoveries of the matrix spike.Method 550.1 was used as a reference.

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